E Polylysine(EP) Cas No. 28211-04-3

Epsilon Polylysine Epsilon Polylysine Introduction Epsilon Polylysine is a naturally produced, cationic homopolymer of 25-30 L-lysine(due to Streptomyces albulus fermentation.), which was connected by ε-amido bond compounded by a e-amido of l-lysine and a e-carboxyl of another l-lysine.

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Epsilon Polylysine

Epsilon Polylysine Introduction

Epsilon Polylysine is a naturally produced, cationic homopolymer of 25-30 L-lysine(due to Streptomyces albulus fermentation.), which was connected by ε-amido bond compounded by a e-amido  of l-lysine and a e-carboxyl of another l-lysine.

Characteristics

◆Various functions: Polylysine molecules are cationic, surface active agents in water due to their positively charged amino groups. This property imports many benefits when used as an ingredient in cosmetics.

◆Very safe.

◆Easy to use: readily soluble in water, highly heat-stable and useable over a wide pH range (5-8).

powder additive

Physical and Chemical Properties
Appearance and Physical State: milky yellow to white

Form at 20°C:powder

Melting Point: 172.8 °C

PH: 5-8
Solubility in Water: soluble

molecular formula:C6H12n+2N2On+1 (n=25~30);

molecular weight:3600~4300 DA


Antibacterial Spectrum

Table 1 ε-PL MIC (minimum inhibition concentration) Test


Different Types of Microorganisms


Bacteric

MIC

μg/ml


pH


Fungi

Aspergillus niger IFO4416

250

5.6

Trichophyton mentagrophytes IFO7522

60

5.6




Yeast

Candida acutus IFO1912

6

5.0

Phaffia rhodozyma IFO10129

12

5.0

Pichia anomala IFO0146

150

5.0

Pichia membranaefaciens IFO0577

< 3

5.0

Rhodotorula lactase IFO1423

25

5.6

Sporobolomyces roseus IFO1037

< 3

5.0

Saccharomyces cerevisiae

50

5.0

Zygosaccharomyces rouxii IFO1130

150

5.6




Gram+ bacteria

Geobacillus stearothermophilus IFO12550

5

7.0

Bacillus coagulans IFO12583

10

7.0

Bacillus subtilis IAM1069

< 3

7.3

Clostridium acetobutylicum IFO13948

32

7.1

Leuconostoc mesenteroides

50

6.0

Lactobacillus brevis IFO3960

10

6.0

L1 plantarum IFO12519

5

6.0

Micrococcus luteus IFO12708

16

7.0

Staphylococcus aureus IFO13276

12

7.0

Streptococcus lactis IFO12546

100

6.0



Gram- bacteria

Raoultella planticola IFO3317

8

7.0

Campylobacter jejuni

100

7.0

Escherichia coli IFO13500

50

7.0

Pseudomonas aeruginosa IFO3923

3

7.0

Salmonella typhymurium




As can be seen, ε-PL has wide range of antimicrobial spectrum with minimum inhibitory concentration of bacteria less than 100μg/ml, and to Fungi, the MIC is higher.

Table-2 Effect of temperature on the Minimum Inhibition Concentration (MIC) of ε-Polylysine against Growth of Escherichia coil

blob.pngTable 3  PH on ε-PL MIC

blob.pngblob.pngΕ-PL can effectively inhibit the growth of undesirable microorganisms in foods, such as bakery food( bread , cake etc.),  beverage( soybean milk, fruit beverage) and meat products.

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